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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Understanding and Editing the Zebrafish Genome.

Gaurav K Varshney¹, Raman Sood¹, Shawn M Burgess¹

¹Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Advances in Genetics

|December 8, 2015

Summary

Genome editing tools like CRISPR/Cas9 have revolutionized zebrafish genetics, enabling targeted mutations. This review details their application and resources for researchers, making complex genetic modifications accessible.

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Area of Science:

Genetics and Genomics
Developmental Biology
Model Organisms

Background:

Zebrafish is a rapidly growing model organism with increasing publication rates.
Historically, the lack of efficient genome editing tools hindered systematic genetic studies in zebrafish.
The advent of sequence-specific nucleases has addressed this limitation.

Purpose of the Study:

To provide a comprehensive review of genome editing tools in zebrafish.
To highlight the application of zinc finger nucleases (ZFNs), TAL-effector nucleases (TALENs), and CRISPR/Cas9 for generating targeted mutations.
To list available resources and online tools for zebrafish genetic research.

Main Methods:

Review of scientific literature on genome editing in zebrafish.

Keywords:

CRISPR/Cas9 Genomics Resources Tal-effector nucleases Targeted mutagenesis Zebrafish Zinc finger nucleases

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Focus on the application of ZFNs, TALENs, and CRISPR/Cas9 technologies.

Compilation of genetic and genomic resources and online design tools.

Main Results:

Genome editing technologies have transformed targeted mutagenesis in zebrafish.
These tools enable the creation of knockout and knock-in mutants efficiently.
Previously resource-intensive targeted mutations are now achievable in most labs.

Conclusions:

Zebrafish genome editing has become significantly more accessible and efficient.
ZFNs, TALENs, and CRISPR/Cas9 are powerful tools for genetic manipulation in zebrafish.
The reviewed resources will aid researchers in designing and implementing genome editing experiments.