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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Related Experiment Video

Updated: Mar 29, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
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High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

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High-throughput analysis and protein engineering using microcapillary arrays.

Bob Chen1, Sungwon Lim1, Arvind Kannan2

  • 1Department of Bioengineering, Stanford University, Stanford, California, USA.

Nature Chemical Biology
|December 8, 2015
PubMed
Summary
This summary is machine-generated.

We developed a versatile microcapillary single-cell analysis and laser extraction (μSCALE) platform for high-throughput protein analysis and engineering. This technology enables rapid discovery and optimization of proteins, including antibodies and enzymes.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • High-throughput screening is crucial for protein engineering and discovery.
  • Existing platforms often lack the ability to perform quantitative measurements and recover live cells.

Purpose of the Study:

  • To introduce and validate the μSCALE platform for massively parallel, quantitative analysis of protein variants.
  • To demonstrate the platform's versatility across different applications and host organisms.

Main Methods:

  • μSCALE spatially segregates single cells in microcapillaries for repeated imaging and expression.
  • Utilizes fluorescent assays for binding-affinity and enzymatic activity measurements.
  • Employs laser-based extraction for recovery of live clones and genetic material.

Main Results:

  • Successfully performed high-throughput analysis on millions of protein variants.
  • Discovered a novel antibody for a cancer target.
  • Evolved a fluorescent protein biosensor and engineered an enzyme.
  • Demonstrated successful application in both yeast and bacterial expression systems.

Conclusions:

  • μSCALE is a flexible and powerful platform for quantitative protein analysis and engineering.
  • Enables rapid discovery and optimization of proteins with diverse applications.
  • Facilitates recovery of valuable biological material for further research.