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Bleeding the laboratory mouse: Not all methods are equal.

Jonathan Hoggatt1, Amber F Hoggatt2, Tiffany A Tate1

  • 1Cancer Center/Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA.

Experimental Hematology
|December 9, 2015
PubMed
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Blood collection methods significantly impact hematologic parameters in mice. Warming mice before tail vein bleeding can standardize results, improving research reproducibility.

Area of Science:

  • * Hematology and immunology research utilizing laboratory mice.
  • * Preclinical research and animal model studies.

Background:

  • * Laboratory mice are crucial models for hematologic studies, often involving nonterminal blood collection.
  • * Mouse physiology presents challenges for blood collection due to small body size, limiting volume, frequency, and site accessibility.
  • * Variability in blood collection techniques across research settings can affect experimental outcomes.

Purpose of the Study:

  • * To investigate the impact of different blood sampling sites and methods on hematologic parameters in mice.
  • * To identify specific blood components affected by sampling techniques.
  • * To provide recommendations for standardizing blood collection in mouse studies.

Main Methods:

  • * Comparison of blood parameters obtained from various sampling sites (e.g., retro-orbital sinus, tail vein, saphenous vein, facial vein, heart).

Related Experiment Videos

  • * Assessment of specific cell populations including total white blood cells, dendritic cells, myeloid-derived suppressor cells, and hematopoietic progenitor cells.
  • * Evaluation of the effect of pre-sampling warming on tail vein blood values.

    • Main Results:

    • * Significant alterations in total white blood cells, dendritic cells, myeloid-derived suppressor cells, and hematopoietic progenitor cells were observed based on sampling site and method.
    • * Warming mice prior to tail vein bleeding demonstrably altered measured blood values.
    • * Inconsistent sampling methods introduce significant variability in hematologic assessments.

    Conclusions:

    • * The site and manner of blood collection critically influence hematologic parameters in laboratory mice.
    • * Standardizing blood collection methods within a study and warming mice before tail vein sampling are recommended to enhance data uniformity.
    • * Detailed reporting of blood collection techniques in publications is essential for inter-laboratory comparisons and reproducibility.