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Updated: Mar 29, 2026
Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
Published on: May 19, 2020
"In-house" production of DNA size marker from a vaccinal Bacillus anthracis strain
Mohammad Sekhavati1, Keyvan Tadayon1, Rainak Ghaderi1
1Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Background And Objectives:
DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings.
Materials And Methods:
Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker.
Results And Conclusion:
The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
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