Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Ubiquinol ameliorates social disruption-induced behavioral changes via modulating inflammatory responses and PPARα activation" [Behav. Brain Res. 508 (2026), 116215].

Behavioural brain research·2026
Same author

Large-scale manufacturing of precisely patterned flexible soft tissue implants with high porosity.

Biofabrication·2026
Same author

Roadmap on biomaterials for women's health.

JPhys materials·2026
Same author

Rapid prediction of cerebral edema on CT scan after traumatic brain injury.

Trauma surgery & acute care open·2026
Same author

Ubiquinol ameliorates social disruption-induced behavioral changes via modulating inflammatory responses and PPARα activation.

Behavioural brain research·2026
Same author

Observation of the Magnon Hall Magnetoresistance Effect.

Physical review letters·2026
Same journal

A Multimodal Strategy for Enhancing Minimally Invasive Ablation of Lung Tumor.

Annals of biomedical engineering·2026
Same journal

Real-Time Soft Tissue Deformation Framework for Haptic-Enabled Robotic Surgical Training in Virtual Reality.

Annals of biomedical engineering·2026
Same journal

Computational Modelling of Selective Capture Mechanisms in Conduction System Pacing.

Annals of biomedical engineering·2026
Same journal

Airborne Ultrasound Surface Motion Camera: Application to Transfer Admittance Chest Imaging.

Annals of biomedical engineering·2026
Same journal

Patient-Specific Adaptation of a Mechano-Regulatory Bone-Healing Model Using Longitudinal Loading Data.

Annals of biomedical engineering·2026
Same journal

Effects of Simulated Body-Mass Reduction on Peak Knee Joint Loads During Daily Functional Activities.

Annals of biomedical engineering·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Hybrid µCT-FMT imaging and image analysis
13:45

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

13.7K

Mesoscopic Fluorescence Molecular Tomography for Evaluating Engineered Tissues.

Mehmet S Ozturk1, Chao-Wei Chen2, Robin Ji1

  • 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.

Annals of Biomedical Engineering
|December 10, 2015
PubMed
Summary
This summary is machine-generated.

Laminar optical tomography (LOT), also known as mesoscopic fluorescence molecular tomography (MFMT), offers non-destructive, depth-resolved imaging for tissue engineering. This technique enables detailed monitoring of cell distribution and maturation in thick constructs, advancing regenerative medicine strategies.

Keywords:
BioprintingFluorescence laminar optical tomography (FLOT)In vivo imagingLaminar optical tomography (LOT)Mesoscopic fluorescence molecular tomography (MFMT)Non-destructive imagingOptical imagingTissue engineering

More Related Videos

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

8.9K
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.0K

Related Experiment Videos

Last Updated: Mar 29, 2026

Hybrid µCT-FMT imaging and image analysis
13:45

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

13.7K
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

8.9K
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.0K

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Optical Imaging

Background:

  • Optimizing regenerative medicine requires non-destructive imaging of engineered tissues to assess cellular structure, function, and molecular expression.
  • Current fluorescence microscopy is limited to superficial tissue examination, hindering the assessment of deep cellular distribution and migration within scaffolds.
  • Advanced imaging techniques are crucial for monitoring cell phenotyping and distribution throughout engineered tissues.

Purpose of the Study:

  • To review the emerging depth-resolved optical mesoscopic imaging technique, laminar optical tomography (LOT) or mesoscopic fluorescence molecular tomography (MFMT).
  • To introduce the physical principles, image formation, and instrumentation of LOT/MFMT systems.
  • To highlight the application of LOT/MFMT in non-destructively imaging cellular distribution and maturation in thick tissue engineering constructs.

Main Methods:

  • Focus on laminar optical tomography (LOT) / mesoscopic fluorescence molecular tomography (MFMT) as a depth-resolved optical mesoscopic imaging technique.
  • Explanation of the physical principle, image formation, and instrumentation of LOT/MFMT systems.
  • Discussion of representative applications in tissue engineering, including cell distribution in hydrogels, bio-printed tissues, and in vivo studies.

Main Results:

  • LOT/MFMT enables longitudinal imaging of cellular distribution in thick tissue engineering constructs.
  • The technique achieves imaging at depths of a few millimeters with relatively high resolution.
  • Demonstrated applications include imaging human mesenchymal stem cells in hydrogels and bio-printed tissues.

Conclusions:

  • Laminar optical tomography (LOT)/mesoscopic fluorescence molecular tomography (MFMT) is a promising technique for non-destructive, depth-resolved imaging in tissue engineering.
  • This method overcomes the limitations of superficial imaging, allowing for comprehensive assessment of engineered tissues.
  • LOT/MFMT facilitates detailed monitoring of cell distribution and maturation, crucial for advancing regenerative medicine.