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Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B

Ji-Shun Zheng1, Meng-Meng Chen2, Hai-Fei Yang2

  • 1Department of Gastroenterology and Infectious Diseases, Anhui Medical University (The Third Affiliated Hospital), Hefei, Anhui, China (mainland).

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
|December 10, 2015
PubMed

Insights

Fecal hepatitis B virus (HBV) DNA and serum HBV DNA show distinct clinical significance in chronic hepatitis B (CHB) patients. Fecal HBV DNA correlates with Enterococcus, while serum HBV DNA relates to liver enzymes and Lactobacillus.

Area of Science:

  • Hepatology
  • Virology
  • Microbiome Research

Background:

  • Serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) levels are linked to liver function in chronic hepatitis B (CHB).
  • The clinical significance of fecal HBV DNA compared to serum HBV DNA in CHB patients remains uninvestigated.

Purpose of the Study:

  • To compare the clinical significance of fecal HBV DNA and serum HBV DNA in CHB patients.
  • To investigate the correlation between fecal and serum HBV DNA levels and host liver function markers and intestinal microbiota.

Main Methods:

  • Collected stool and serum samples from 66 CHB patients.
  • Quantified fecal HBV DNA, serum HBV DNA, and intestinal microbiota DNA using real-time quantitative PCR.
  • Analyzed liver function markers and HBeAg status.

Main Results:

  • A positive correlation was found between fecal and serum HBV DNA (r=0.57, P=0.001).
  • Fecal HBV DNA was higher in HBeAg-positive patients and negatively correlated with alkaline phosphatase (ALP) and total bilirubin (TBIL).
  • Fecal HBV DNA positively correlated with Enterococcus, while serum HBV DNA negatively correlated with alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Lactobacillus.

Conclusions:

  • Fecal and serum HBV DNA exhibit different clinical implications in CHB patients.
  • Fecal HBV DNA may be associated with alterations in Enterococcus populations.
  • Serum HBV DNA is not associated with Enterococcus changes, suggesting distinct pathogenic roles.

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