Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.5K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two atypical resistance proteins confer plant resistance to Melanaphis sorghi in sorghum.

Plant communications·2026
Same author

Neural Regulation of Cardiac Arrhythmias: From the Brain-Heart Axis to Emerging Precision Therapies.

Research (Washington, D.C.)·2026
Same author

Activated CC<sub>G10</sub>-NLR: Octameric resistosome driving sustained calcium influx.

Journal of integrative plant biology·2026
Same author

Genomic diversity at the <i>Glu-D1</i> locus in <i>Aegilops tauschii</i> reveals the origin of elite high-molecular-weight glutenin genes in bread wheat (<i>Triticum aestivum</i>).

Plant diversity·2026
Same author

Transcriptome analysis and characterization of cathepsin B-like protein in <i>Melanaphis sorghi</i> in response to resistant sorghum.

Molecular breeding : new strategies in plant improvement·2026
Same author

LOX-A4 shapes Triticum urartu gene pools and contributions to the A subgenome of polyploid wheat.

Nature communications·2026

Related Experiment Video

Updated: Mar 29, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

8.0K

Single-molecule real-time transcript sequencing facilitates common wheat genome annotation and grain transcriptome

Lingli Dong1, Hongfang Liu2, Juncheng Zhang3

  • 1The State Key Laboratory of Plant cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. lldong@genetics.ac.cn.

BMC Genomics
|December 10, 2015
PubMed
Summary

PacBio sequencing of wheat caryopses revealed thousands of new genes and transcripts, significantly improving genome annotation. This research enhances understanding of wheat gene regulation and quality traits.

More Related Videos

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

12.4K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.6K

Related Experiment Videos

Last Updated: Mar 29, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

8.0K
Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

12.4K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.6K

Area of Science:

  • Plant Genomics
  • Transcriptomics
  • Molecular Biology

Background:

  • The hexaploid wheat genome's complexity hinders genomic studies.
  • Previous research faced challenges in comprehensive wheat gene annotation.

Purpose of the Study:

  • To improve common wheat (Triticum aestivum) genome annotation.
  • To advance grain transcriptome research using novel sequencing data.

Main Methods:

  • Utilized single-molecule real-time (SMRT) sequencing technology (PacBio RSII).
  • Analyzed transcripts from developing wheat caryopses.
  • Mapped full-length non-chimeric (FLNC) reads to the wheat genome.

Main Results:

  • Identified 91,881 high-quality FLNC reads representing 22,768 unique transcripts.
  • Discovered 3026 new genes and 9591 newly discovered transcripts.
  • Found 6030 differentially regulated genes and 72 gluten gene transcripts during caryopsis development.

Conclusions:

  • PacBio transcript sequencing effectively improves wheat genome annotation by revealing previously undiscovered loci and transcripts.
  • The generated data resource supports future structural genomics and grain transcriptome studies in wheat.