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Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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Protein Connectivity in Chemotaxis Receptor Complexes.

Stephan Eismann1,2, Robert G Endres2

  • 1Department of Physics and Astronomy, University of Heidelberg, Heidelberg, Germany.

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|December 10, 2015
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Summary

Bacteria use chemotaxis to find food and avoid toxins. This study models receptor interactions, revealing how CheA and CheW proteins control bacterial sensing sensitivity and cooperativity, offering evolutionary insights.

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Area of Science:

  • Microbiology
  • Biophysics
  • Systems Biology

Background:

  • Bacterial chemotaxis relies on a sensitive sensory system for nutrient detection and avoidance of repellents.
  • Receptor interactions at cell poles are critical for chemotaxis sensitivity, but the exact coupling mechanism involving CheA and CheW is unclear.

Purpose of the Study:

  • To model the receptor linkage mechanism in bacterial chemotaxis.
  • To investigate the impact of CheA and CheW expression levels on the sensing behavior of mixed receptor clusters.

Main Methods:

  • Utilized a statistical-mechanics approach informed by nanodisc and electron cryotomography experiments.
  • Compared model predictions with in vivo Förster resonance energy transfer (FRET) measurements.

Main Results:

  • Receptor cooperativity is minimally affected by receptor-methylation levels.
  • Receptor cooperativity decreases with increasing CheA levels but increases with increasing CheW levels.
  • The model explains the non-intuitive effects of CheA and CheW on receptor cooperativity.

Conclusions:

  • CheA and CheW play distinct, crucial roles in modulating bacterial chemotaxis receptor cooperativity.
  • An evolutionary advantage may explain the existence of CheW-only linker structures.