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Magnetite loaded Polypeptide-PLGA multifunctional microbubbles for dual-mode US/MR imaging
Ying Sun1, Yunkai Zhu2, Can Huang1
1State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200032, People's Republic of China.
Abstract:
Magnetite loaded Polypeptide-PLGA multifunctional microbubbles (Fe3O4 /Polypeptide-PLGA MMBs) that show superparamagnetic properties were prepared by a modified double emulsion method and employed as imaging agent for dual-mode Ultrasound/Magnetic resonance (US/MR) imaging of prostatic cancer. The successful synthesis of MMBs was determined by Fourier Transform Infrared Spectrometer (FTIR), X-ray diffraction (XRD), Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), Atomic Absorption Spectroscopy (AAS) and vibrating sample magnetometer (VSM). The as-prepared MMBs had a diameter of 700 nm and were quite safe as confirmed by MTT assays. Prussian Blue Staining showed that targeted Fe3O4 /Polypeptide-PLGA MMBs enhanced the cellular uptake efficiency. In cell attachment study, adherence of MMBs was significantly higher to LNCaP cells compared with negative control PC3 cells. The in vitro results demonstrated that these MMBs could enhance both US and MR imaging of prostatic cancer.
Insights
New multifunctional microbubbles loaded with magnetite show superparamagnetic properties for enhanced dual-mode Ultrasound/Magnetic Resonance imaging of prostate cancer. These microbubbles demonstrate improved cellular uptake and safety for potential clinical applications.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Medical Imaging
Background:
- Prostate cancer diagnosis requires advanced imaging techniques.
- Multimodal imaging agents can improve diagnostic accuracy.
- Microbubbles offer potential as contrast agents for ultrasound and MRI.
Purpose of the Study:
- To synthesize and characterize magnetite-loaded Polypeptide-PLGA multifunctional microbubbles (Fe3O4/Polypeptide-PLGA MMBs).
- To evaluate the potential of these MMBs as a dual-mode Ultrasound/Magnetic Resonance (US/MR) imaging agent for prostate cancer.
- To assess the safety and cellular uptake efficiency of the synthesized MMBs.
Main Methods:
- Fe3O4/Polypeptide-PLGA MMBs were prepared using a modified double emulsion method.
- Synthesis was confirmed using FTIR, XRD, TEM, SEM, AAS, and VSM.
- MTT assays were used to evaluate safety, and Prussian Blue Staining assessed cellular uptake.
Main Results:
- Superparamagnetic MMBs with a diameter of 700 nm were successfully synthesized.
- MTT assays confirmed the safety of the MMBs.
- Targeted MMBs showed enhanced cellular uptake and significantly higher adherence to LNCaP cells compared to PC3 cells.
Conclusions:
- Fe3O4/Polypeptide-PLGA MMBs are safe and effective for dual-mode US/MR imaging of prostate cancer.
- The MMBs demonstrate enhanced cellular uptake and targeting capabilities.
- These findings suggest potential for improved prostate cancer diagnosis using this novel imaging agent.
