Related Experiment Video
Updated: Mar 29, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[Improving isoprene production by engineered heterologous mevalonate pathway in Escherichia coli]
Abstract:
Isoprene is an important precursor of synthetic rubber material. In our previous study, metabolic engineered Escherichia coli strain (BW-01) was constructed and used to produce isoprene. Based on the theory of protein budget, using synthetic biology strategies including the increased copy number of genes and rare codons, we regulated the expression of key enzyme to improve isoprene production in Escherichia coli strain. Under shake-flask conditions, isoprene productivity of the engineered strain (BW-07) increased by 73% compared with BW-01, reached 761.1 mg/L. It provides a reference for further studies.
More Related Videos
10:41The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
Published on: January 13, 2013
08:37Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Related Concept Videos
Production of Pharmaceuticals
Bioreactor Controls-III
Biosynthesis in Bacteria
Biosynthesis of Lipids
Production of Antibiotics
Amino Acid Biosynthetic Pathways