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Rutin ameliorates methotrexate induced hepatic injury in rats
Esra Erdogan1, Yasin Ilgaz1, Pinar Naile Gurgor2
1Gulhane Military Medical Academy, Ankara, Turkey.
Purpose:
To investigate the possible protective effect of rutin on methotrexate induced hepatotoxicity in rats.
Methods:
Twenty-two rats were divided into three experimental groups; Control-saline, Mtx, Mtx+Rutin. Hepatic tissue was taken for histological assessment and biochemical assays. Oxidative stress parameters malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were investigated. Liver markers aspartate aminotransferase (AST), alanine aminotransferase (ALT) were analyzed in serum.
Results:
Mtx+Rutin group showed lower histological injury compared to Mtx group, MDA and ALT levels were increased, while SOD and GSH-Px were decreased in Mtx group compared with Control-saline group. MDA and ALT levels were increased, while SOD and GSH-Px were decreased in Mtx group, compared with Mtx +Rutin group. Serum AST levels were similar among the groups.
Conclusion:
Rutin may be a potential adjuvant drug to reduce the hepatic side effects observed during Mtx therapy for various clinical conditions.
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