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[Transfected miR-1908 inhibits renal fibrosis via targeting transforming growth factor beta 1]
Fei Xie1, Xiaoshun Li2, Chan Wei2
1Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China. *Corresponding author,
Objective:
To explore the regulatory role of miR-1908 in renal fibrosis.
Methods:
The level of miR-1908 and transforming growth factor beta 1 (TGF-β1) mRNA during renal fibrosis were detected with real-time quantitative PCR. Bioinformatics and luciferase reporter gene analyses were applied to determine the targeting relationship between miR-1908 and TGF-β1 mRNA. After primary human renal interstitial fibroblasts were transfected with miR-1908 adenoviral expression vector in vitro, Western blotting was used to detect the protein levels of TGF-β1, smad2/3 and matrix metalloproteinase 2 (MMP-2) in the cells. Six weeks after intraperitoneal injection of miR-1908 adenoviral vector, the renal tissue sections of the renal fibrosis mouse models were stained with Masson staining.
Results:
Human miR-1908 showed a gradually decreasing expression during renal fibrosis process, which was completely contrary to the changes of TGF-β1 mRNA. Overexpression of miR-1908 suppressed the expressions of TGF-β1, smad2/3 and MMP-2 in human primary renal interstitial cells. The renal fibrosis was significantly relieved in the mice injected with miR-1908 adenovirus vector injection compared with the ones without injection.
Conclusion:
miR-1908 could inhibit renal fibrosis through targeting TGF-β1.
Insights
MicroRNA-1908 (miR-1908) inhibits renal fibrosis by targeting transforming growth factor beta 1 (TGF-β1). This study reveals miR-1908 as a potential therapeutic target for kidney fibrosis.
Area of Science:
- Molecular Biology
- Renal Pathophysiology
- MicroRNA Therapeutics
Context:
- Renal fibrosis is a common pathway for progressive kidney diseases.
- MicroRNAs (miRNAs) play crucial roles in regulating cellular processes, including fibrosis.
- Identifying novel miRNA regulators is essential for developing targeted therapies.
Purpose:
- To investigate the role of miR-1908 in the pathogenesis of renal fibrosis.
- To determine if miR-1908 targets transforming growth factor beta 1 (TGF-β1).
- To evaluate the therapeutic potential of miR-1908 in a mouse model of renal fibrosis.
Summary:
- miR-1908 expression decreased during renal fibrosis, inversely correlating with TGF-β1 mRNA levels.
- Overexpressing miR-1908 in human renal interstitial fibroblasts reduced TGF-β1, smad2/3, and MMP-2 expression.
- In vivo administration of miR-1908 adenovirus vector significantly ameliorated renal fibrosis in mice.
Impact:
- miR-1908 acts as a suppressor of renal fibrosis.
- The mechanism involves targeting TGF-β1 signaling pathway.
- miR-1908 represents a promising therapeutic candidate for treating kidney fibrosis.
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