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Updated: Mar 29, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Microsatellite markers isolated from Cabomba aquatica s.l. (Cabombaceae) from an enriched genomic library
Tiago D M Barbosa1, Rafaela J Trad1, Miklos M Bajay2
1Departamento de Biologia Vegetal, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, São Paulo, Brazil.
Premise Of The Study:
Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters.
Methods And Results:
Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Viruá population.
Conclusions:
The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.
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