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Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
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Controlling the response to DNA damage by the APC/C-Cdh1
H Rudolf de Boer1, S Guerrero Llobet1, Marcel A T M van Vugt2
1Department of Medical Oncology, Cancer Research Center Groningen, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Cellular and Molecular Life Sciences : CMLS
|December 10, 2015
Summary
The anaphase-promoting complex/cyclosome (APC/C) with its adaptor protein Cdh1 is activated by DNA damage. This APC/C-Cdh1 complex plays a crucial role in cell cycle regulation and fate determination following DNA damage.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Cell cycle progression relies on cyclin/cyclin-dependent kinase complexes.
- The anaphase-promoting complex/cyclosome (APC/C) is a key regulator of mitotic cyclins.
- APC/C activation by Cdc20 or Cdh1 targets mitotic regulators for degradation, ensuring proper mitosis.
Purpose of the Study:
- To review the regulation of APC/C in response to DNA damage.
- To explore the functions of APC/C-Cdh1 activation upon DNA damage.
- To discuss the role of APC/C-Cdh1 in controlling cell fate with persistent DNA damage.
Main Methods:
- Literature review of in vitro and in vivo studies.
- Analysis of genetic studies on Cdh1-deficient cells.
- Synthesis of current data on APC/C regulation and function.
Main Results:
- APC/C-Cdh1 is activated in G2 phase cells following DNA damage.
- Cdh1 deficiency leads to impaired DNA repair and aberrant cell cycle checkpoints.
- APC/C-Cdh1 activation is a critical response to DNA damage.
Conclusions:
- APC/C-Cdh1 activation is a significant event in the DNA damage response.
- APC/C-Cdh1 influences cell cycle checkpoints and DNA repair.
- Understanding APC/C-Cdh1 function is vital for comprehending cell fate decisions under genotoxic stress.
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