Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uptake of Quitline Telephone Counselling by Women Who Smoke During Pregnancy.

The Australian & New Zealand journal of obstetrics & gynaecology·2025
Same author

A systematic review of interventions to increase the use of smoking cessation services for women who smoke during pregnancy.

The Australian & New Zealand journal of obstetrics & gynaecology·2023
Same author

Looking to the future: Big data drives biomedical research.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2022
Same author

Neonatal near miss: A review of current definitions and the need for standardisation.

The Australian & New Zealand journal of obstetrics & gynaecology·2022
Same author

Effectiveness of neonatal "near miss" audits in reducing perinatal morbidity and mortality: a systematic review protocol.

JBI evidence synthesis·2021
Same author

Learning how to teach science with big data.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2021
Same journal

Correction to: Determination of Alkaloids in Mitragyna speciosa (Kratom) Raw Materials and Dietary Supplements by HPLC-UV: Single-Laboratory Validation, First Action 2017.14.

Journal of AOAC International·2026
Same journal

Single-Laboratory Validation of Simultaneous Determination of Aflatoxins in Nutraceuticals following immune-affinity Column Cleanup and Liquid Chromatography Tandem Mass Spectrometry Analysis.

Journal of AOAC International·2026
Same journal

Determination of Bromoform in Seaweed, Oil, and Animal Feed by GC-MS/MS: AOAC Official Method 2026.01, First Action.

Journal of AOAC International·2026
Same journal

ICRF-Assisted Box-Behnken Design and Optimization for Rapid UPLC-PDA Determination of Dorzolamide Hydrochloride and Timolol Maleate in an Ophthalmic Preparation.

Journal of AOAC International·2026
Same journal

Advancing PFAS Analysis Through Scientific Publication.

Journal of AOAC International·2026
Same journal

A Green UPLC-UV Method for L-Ascorbic Acid Determination Based on a Biodegradable Chelating Agent and Synergistic Hydrophobic-Electrostatic Interactions.

Journal of AOAC International·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

18.9K

QuickTox™ Kit for QuickScan Aflatoxin FREE.

Sergiusz Polakowski, Russell W Roberts, Keith Tanguay

    Journal of AOAC International
    |December 15, 2015
    PubMed
    Summary
    This summary is machine-generated.

    The QuickTox Kit accurately quantifies total aflatoxins in various food matrices using lateral flow technology. This method demonstrates good performance across multiple sample types, with minor deviations noted only in robustness testing.

    More Related Videos

    Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
    10:24

    Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

    Published on: April 19, 2024

    1.8K
    RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
    09:44

    RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

    Published on: December 21, 2015

    21.7K

    Related Experiment Videos

    Last Updated: Mar 29, 2026

    Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
    10:01

    Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

    Published on: April 23, 2012

    18.9K
    Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
    10:24

    Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

    Published on: April 19, 2024

    1.8K
    RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
    09:44

    RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

    Published on: December 21, 2015

    21.7K

    Area of Science:

    • Food safety and analytical chemistry
    • Mycotoxin detection and quantification
    • Lateral flow immunoassay development

    Background:

    • Aflatoxins are toxic secondary metabolites produced by Aspergillus fungi, posing significant health risks.
    • Accurate and rapid detection of aflatoxins in diverse food commodities is crucial for regulatory compliance and consumer protection.
    • Existing methods for aflatoxin analysis can be time-consuming, require specialized equipment, or involve hazardous solvents.

    Purpose of the Study:

    • To evaluate the performance of the QuickTox Kit for QuickScan Aflatoxin FREE for quantitative determination of total aflatoxins.
    • To assess the assay's suitability for various food matrices using optimized extraction protocols.
    • To validate the assay's accuracy, linearity, selectivity, and robustness against established criteria.

    Main Methods:

    • Utilized competitive lateral flow technology with a reader-based system for quantitative aflatoxin determination.
    • Employed matrix-specific extraction protocols: aqueous for corn/wheat, 50% ethanol for oats/sorghum/barley, and 80% ethanol for peanut products.
    • Conducted performance evaluations including linearity, selectivity, matrix effects, lot consistency, and robustness studies.

    Main Results:

    • The assay demonstrated good performance across multiple matrices, with results generally within acceptance criteria.
    • Robustness studies showed deviations when sample volume and assay time were simultaneously altered.
    • Sensitivity was highest for Aflatoxin B1, B2, and G1, with G2 sensitivity at 64% of B1; other mycotoxins did not interfere.
    • Linear dose response observed over 0-100 ppb range (R(2) > 0.93), with acceptable relative standard deviation values.

    Conclusions:

    • The QuickTox Kit provides a reliable method for quantitative total aflatoxin determination in various food matrices.
    • The assay's performance is validated for key parameters, supporting its use in routine analysis.
    • Optimized extraction procedures enhance usability and reduce solvent consumption for specific commodities.