Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlative Fluorescent Scanning Probe Nanotomography Used to Study the Intracellular Distribution of Doxorubicin in MCF-7 Human Breast Adenocarcinoma Cells.

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections·2023
Same author

Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.

Biomedical materials (Bristol, England)·2019
Same author

Effect of lipopeptide structure on gene delivery system properties: Evaluation in 2D and 3D in vitro models.

Colloids and surfaces. B, Biointerfaces·2018
Same author

[Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same author

[Biodegradablescaffolds based on chitosan: Preparation, properties, and use for the cultivation of animal cells].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same author

[A technique for X-ray protection monitoring in electron accelerators with regard for the activity mode].

Meditsinskaia tekhnika·2014

Related Experiment Video

Updated: Mar 29, 2026

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology
05:21

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

3.6K

Polylactide-based microspheres prepared using solid-state copolymerized chitosan and d,l-lactide.

T S Demina1, T A Akopova1, L V Vladimirov2

  • 1Enikolopov Institute of Synthetic Polymer Materials, Russian Academy of Sciences, Profsoyuznaya Str., 70, 117393 Moscow, Russia.

Materials Science & Engineering. C, Materials for Biological Applications
|December 15, 2015
PubMed
Summary

Novel amphiphilic chitosan-g-poly(d,l-lactide) copolymers were synthesized for tissue engineering microspheres. These biodegradable copolymers replace traditional emulsifiers and enhance cell adhesion for improved biomaterial development.

Keywords:
ChitosanGraft copolymersMicroparticlesOil/water emulsionSolid-state reactive blendingTissue engineeringd,l-Lactide

More Related Videos

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
09:39

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

17.5K
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

4.1K

Related Experiment Videos

Last Updated: Mar 29, 2026

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology
05:21

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

3.6K
Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
09:39

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

17.5K
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

4.1K

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • Chitosan-g-poly(d,l-lactide) copolymers offer amphiphilic properties suitable for biomaterial applications.
  • Traditional emulsifiers in microparticle preparation are often non-degradable, posing limitations for tissue engineering scaffolds.
  • Surface modification of microspheres is crucial for enhancing cell adhesion and integration in tissue regeneration.

Purpose of the Study:

  • To synthesize amphiphilic chitosan-g-poly(d,l-lactide) copolymers using solid-state mechanochemical copolymerization.
  • To utilize these novel copolymers as biodegradable stabilizers in polyester-based microsphere fabrication for tissue engineering.
  • To functionalize the microsphere surface with positively charged segments to promote cell adhesion.

Main Methods:

  • Solid-state reactive blending (SSRB) via low-temperature co-extrusion for copolymer synthesis.
  • Oil/water emulsion evaporation technique for microsphere preparation.
  • Characterization of microsphere size distribution, surface morphology, and yield based on copolymer composition.

Main Results:

  • Successful synthesis of amphiphilic chitosan-g-poly(d,l-lactide) copolymers.
  • Demonstrated efficacy of copolymers as biodegradable emulsifiers, replacing non-degradable alternatives.
  • Tailored copolymer composition influenced microsphere characteristics and surface properties for enhanced cell adhesion.

Conclusions:

  • Amphiphilic chitosan-g-poly(d,l-lactide) copolymers are effective for creating biodegradable microspheres for tissue engineering.
  • The developed copolymers provide a sustainable alternative to conventional emulsifiers in microparticle fabrication.
  • Surface functionalization with charged segments promotes cell adhesion, highlighting potential for advanced tissue regeneration applications.