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Updated: Mar 29, 2026
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
Published on: November 10, 2016
A high sensitive label-free immunosensor for the determination of human serum IgG using overoxidized polypyrrole
Mahmoud Amouzadeh Tabrizi1, Mojtaba Shamsipur2, Ali Mostafaie3
1Department of Chemistry, Razi University, Kermanshah, Iran; Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract:
Herein, an electrochemical human immunoglobulin G (HIgG) immunosensor was fabricated using overoxidized polypyrrole decorated with gold nanoparticle (OPPy-Aunano) modified screen printed electrode (OPPy-Aunano/SPE). The results clearly demonstrate that OPPy-Aunano film is very permeable to [Fe(CN)6](3-/4-). Therefore, the fabrication of immunosensor based on permeability of OPPy-Aunano film to [Fe(CN)6](3-/4-) probe is a novel strategy for the fabrication of label free electrochemical immunosensor. The electrochemical impedance spectroscopy (EIS) techniques are used for the determination of HIgG in the concentration range 0.5 to 125.0ngmL(-1) and with a limit of detection of 0.02ngmL(-1), respectively.
