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Updated: Mar 29, 2026

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Published on: December 3, 2020
Bioanalytical method validation for the simultaneous determination of ceftazidime and avibactam in rat plasma
Marie-Eve Beaudoin1, Eric T Gangl1
1DMPK Oncology iMED, AstraZeneca, Waltham, MA, USA.
Background:
Combination therapies have gained momentum in the disease management strategies of various indications. While it is challenging and more time consuming to develop a combined analytical method, the strategy of simultaneous analysis offers significant advantages in terms of efficiency and cost-effectiveness.
Results:
Due to a significant difference in efficacious dose for ceftazidime and avibactam, the calibration ranges validated in this paper were set to 0.05-50 μg/ml for ceftazidime and 0.005-5.0 μg/ml for avibactam. Interday results of ceftazidime were within 8% for accuracy and 9% for precision and within 9% for both accuracy and precision of avibactam.
Conclusion:
A sensitive and selective LC-MS/MS method was developed for the simultaneous quantification of ceftazidime and avibactam in rat plasma.
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