Related Experiment Video
Updated: Aug 16, 2026

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
Published on: December 29, 2017
Comparative analysis of alternating purine-pyrimidine sites in yeast chromosomes
F Rollo1, A Vaughan Martini, A Martini
1Dipartimento di Biologia Cellulare, Camerino.
Abstract:
It is known that under some circumstances DNA sequences made up of purine and pyrimidines in repeated alternation can undergo a transition from the right-handed to a left handed helical configuration which has been called Z-DNA. We have analyzed yeast chromosomal DNAs for the presence of alternating purine-pyrimidine stretches using a new strategy. Our strategy takes advantage of pulsed field gel electrophoresis (PFGE) to separate yeast chromosomal DNAs. They are subsequently analyzed by molecular hybridization with radioactive (dC-dA)n.(dG-dT)n or (dG-dC)n.(dC-dG)n probes, autoradiography and densitometric scanning. Preliminary results obtained by the application of the new method to Saccharomyces cerevisiae are described.
More Related Videos
Related Concept Videos
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Meiosis vs. Mitosis
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...

