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Related Concept Videos

Introduction to the Human Microbiota01:22

Introduction to the Human Microbiota

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Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
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Development of Human Microbiota01:30

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The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
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Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
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The microbiome quality control project: baseline study design and future directions.

Rashmi Sinha1, Christian C Abnet2, Owen White3

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Microbiome research faces reproducibility challenges due to measurement variations. The Microbiome Quality Control (MBQC) project aims to identify and quantify these variations to improve study reliability.

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Area of Science:

  • Microbiome research
  • Biomedical research
  • Metagenomics

Background:

  • Microbiome studies have rapidly expanded, but suffer from poor reproducibility across different laboratories and investigations.
  • Systematic assessment of measurement variation sources in microbiome research is lacking.
  • Reproducibility concerns are rising within the broader scientific community.

Purpose of the Study:

  • To identify sources of variation in microbiome studies.
  • To quantify the magnitude of these variations.
  • To evaluate control strategies for enhancing microbiome study reproducibility.

Main Methods:

  • The Microbiome Quality Control (MBQC) project was initiated.
  • A baseline study was conducted.
  • A workshop was held to discuss findings and strategies.

Main Results:

  • The first MBQC baseline study and workshop have been completed.
  • Initial findings on variation sources and magnitudes are reported.
  • The utility of positive and negative controls is being assessed.

Conclusions:

  • Addressing measurement variation is critical for advancing microbiome research.
  • Standardized protocols and robust controls are needed.
  • The MBQC project provides a framework for improving microbiome study quality.