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Genome editing in zebrafish: a practical overview.

Robert Sertori, Monique Trengove, Faiza Basheer

    Briefings in Functional Genomics
    |December 15, 2015
    PubMed
    Summary

    Zebrafish are a key model for studying vertebrate development. Genome editing tools offer precise ways to engineer the zebrafish genome, advancing research into development and disease.

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    Area of Science:

    • * Developmental Biology
    • * Genomics
    • * Zebrafish Models

    Background:

    • * Zebrafish are a widely used vertebrate model organism.
    • * Genetic manipulation is crucial for understanding development and disease.
    • * Genome editing technologies have advanced significantly.

    Purpose of the Study:

    • * To provide a practical overview of genome editing in zebrafish research.
    • * To highlight the utility of precise genome engineering in this model organism.
    • * To discuss strategies for introducing and screening genetic modifications.

    Main Methods:

    • * Review of current genome editing techniques (e.g., CRISPR-Cas9).
    • * Discussion of methods for delivering editing components into zebrafish embryos.
    Keywords:
    CRISPRHDRTALENsgenome editingzebrafishzinc finger nucleases

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  • * Examination of screening strategies for identifying desired genetic alterations.

    • Main Results:

    • * Genome editing offers unprecedented precision in modifying the zebrafish genome.
    • * This technology enhances the study of gene function in development and disease.
    • * Various strategies exist for efficient genome engineering and screening in zebrafish.

    Conclusions:

    • * Genome editing has revolutionized zebrafish research capabilities.
    • * Precise genetic engineering in zebrafish accelerates the understanding of vertebrate development and disease mechanisms.
    • * The review offers practical insights for researchers utilizing these advanced techniques.