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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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A fluorescent aptasensor using double-stranded DNA/graphene oxide as the indicator probe.

Xiao-Jing Xing¹, Wan-Lu Xiao², Xue-Guo Liu³

¹Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, China; College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang 473061, China.

Biosensors & Bioelectronics

|December 15, 2015

Summary

This summary is machine-generated.

We developed a novel fluorescent aptasensor using double-stranded DNA/graphene oxide and exonuclease I for sensitive adenosine detection. This label-free platform offers improved performance and potential for diagnosing adenosine-related diseases.

Keywords:

Exonuclease I Fluorescent aptasensor Graphene oxide

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

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Published on: March 21, 2018

Area of Science:

Biomedical Engineering
Nanotechnology
Molecular Diagnostics

Background:

Aptasensors offer sensitive detection of biomarkers.
Graphene oxide (GO) and exonuclease I (Exo I) are utilized in biosensing platforms.
Label-free detection methods are desirable for simplifying biosensor design.

Purpose of the Study:

To develop a novel fluorescent aptasensor for adenosine (AD) detection.
To utilize double-stranded DNA (dsDNA)/graphene oxide (GO) as a signal probe combined with Exo I activity.
To achieve a label-free and highly sensitive detection platform.

Main Methods:

Aptasensor design utilizing dsDNA/GO as signal probe and Exo I activity.
Exploiting differential affinities of aptamer for target vs. competitor and dsDNA/mononucleotides for GO.
Adenosine (AD) as a model analyte for performance evaluation.

Main Results:

The aptasensor demonstrated a lower dissociation constant (Kd = 311.0 µM) and detection limit (LOD = 3.1 µM) compared to traditional methods.
High specificity and successful detection of AD in human serum were achieved.
Optimizing the competitor sequence enhanced the sensor's sensitivity.

Conclusions:

The developed fluorescent aptasensor is a promising tool for diagnosing AD-relevant diseases.
The label-free platform offers advantages over traditional dye-labeled systems.
The study extends the application of dsDNA/GO complexes in biochemical and biomedical studies.