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Updated: Mar 28, 2026

Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Published on: February 22, 2020
Alginate Biosynthesis Factories in Pseudomonas fluorescens: Localization and Correlation with Alginate Production
Susan Maleki1, Eivind Almaas1, Sergey Zotchev1
1Department of Biotechnology, NTNU Norwegian University of Science and Technology, Trondheim, Norway.
Pseudomonas fluorescens produces alginate via a membrane-spanning complex. Alginate production is mainly determined by precursor availability and growth phase, not the number of alginate factories.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Pseudomonas fluorescens synthesizes alginate, a crucial exopolysaccharide for medical and industrial applications.
- Alginate production involves a complex of proteins spanning bacterial membranes.
Purpose of the Study:
- To develop a method for detecting the AlgE porin involved in alginate secretion.
- To investigate the localization and regulation of alginate biosynthesis and secretion machinery.
Main Methods:
- Immunogold labeling and transmission electron microscopy were used to detect the AlgE porin.
- Clustering analysis was employed to study the distribution of alginate complexes.
Main Results:
- The localization of AlgE protein was dependent on the presence of other proteins in the multiprotein complex.
- No correlation was found between alginate factory numbers and alginate production levels.
- Alginate production rate appears primarily regulated by precursor availability and bacterial growth phase.
Conclusions:
- Alginate production in Pseudomonas fluorescens is influenced by precursor availability and growth phase.
- The distribution of alginate synthesis complexes on the cell surface is non-random.
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