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Updated: Mar 28, 2026

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
Published on: September 15, 2014
Definition of a Bidirectional Activity-Dependent Pathway Involving BDNF and Narp
Abigail Mariga1, Juliane Glaser2, Leo Mathias3
1Cell and Molecular Biology Program, New York University, Langone Medical Center, New York, NY 10016, USA; Department of Cell Biology, Skirball Institute of Bimolecular Medicine, NYU Langone Medical Center, New York, NY 10016, USA.
Abstract:
One of the cardinal features of neural development and adult plasticity is the contribution of activity-dependent signaling pathways. However, the interrelationships between different activity-dependent genes are not well understood. The immediate early gene neuronal-activity-regulated pentraxin (NPTX2 or Narp) encodes a protein that has been associated with excitatory synaptogenesis, AMPA receptor aggregation, and the onset of critical periods. Here, we show that Narp is a direct transcriptional target of brain-derived neurotrophic factor (BDNF), another highly regulated activity-dependent gene involved in synaptic plasticity. Unexpectedly, Narp is bidirectionally regulated by BDNF. Acute BDNF withdrawal results in downregulation of Narp, whereas transcription of Narp is greatly enhanced by BDNF. Furthermore, our results show that BDNF directly regulates Narp to mediate glutamatergic transmission and mossy fiber plasticity. Hence, Narp serves as a significant epistatic target of BDNF to regulate synaptic plasticity during periods of dynamic activity.
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