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MiRNA-323-5p Promotes U373 Cell Apoptosis by Reducing IGF-1R

Hong-An Yang1, Xiang Wang2, Feng Ding3

  • 1Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
|December 15, 2015
PubMed

Insights

MicroRNA-323-5p inhibits human glioma U373 cell growth and proliferation while promoting apoptosis. This effect is mediated by the down-regulation of the insulin-like growth factor 1 receptor (IGF-1R).

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • MicroRNAs regulate mammalian cell growth, proliferation, and apoptosis by controlling gene expression.
  • MiRNA-323-5p is implicated in cell growth and death, but its specific role in human cerebral glioma U373 cells requires clarification.

Purpose of the Study:

  • To investigate the regulatory function of miRNA-323-5p in human glioma U373 cell growth, proliferation, and apoptosis.
  • To elucidate the molecular mechanism underlying miRNA-323-5p's action in these cells.

Main Methods:

  • Human cerebral glioma U373 cells were used as a model system.
  • Liposome-mediated transfection (Lipofectamine 2000) was employed to over-express miRNA-323-5p or silence IGF-1R using siRNA.
  • MTT assay, flow cytometry, RT-PCR, and Western blotting were utilized to assess cell behavior and molecular changes.

Main Results:

  • Over-expression of miRNA-323-5p significantly inhibited U373 cell growth and proliferation and induced apoptosis.
  • MiRNA-323-5p over-expression led to a reduction in insulin-like growth factor 1 receptor (IGF-1R) levels.
  • Knockdown of IGF-1R enhanced apoptosis in miRNA-323-5p transfected cells, while IGF-1R over-expression counteracted miRNA-323-5p-induced apoptosis.

Conclusions:

  • MiRNA-323-5p acts as an inhibitor of human cerebral glioma U373 cell proliferation.
  • MiRNA-323-5p promotes apoptosis in U373 cells through the down-regulation of IGF-1R.
  • IGF-1R is a key molecular target mediating the effects of miRNA-323-5p on glioma cell behavior.

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