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Updated: Aug 11, 2026
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Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
Published on: December 18, 2010
[Flow cytometric analysis of oncogene products]
1Dept. of Pediatrics, Kumamoto University Medical School.
Gan to Kagaku Ryoho. Cancer & Chemotherapy
|July 1, 1989
Summary
High levels of H-ras p21 in multiple myeloma (MM) cells, detected by flow cytometry (FCM), indicate a poor prognosis. This assay aids in understanding tumor cell pathophysiology and proliferation.
Area of Science:
- Oncology
- Immunology
- Cell Biology
Context:
- Flow cytometry (FCM) enables novel cell biological investigations of human neoplasia.
- Oncogene product analysis offers new diagnostic and prognostic insights.
Purpose:
- To review the application of FCM for oncogene product analysis in human neoplasia.
- To investigate the prognostic significance of H-ras p21 expression in multiple myeloma (MM) using dual FCM.
Summary:
- Dual H-ras p21/DNA FCM analysis of DNA-aneuploid MM patients revealed that high H-ras p21 expression correlates with poor prognosis.
- The study suggests H-ras p21 expression in MM is linked to cell proliferation/differentiation rather than oncogenic mutation.
- Multiparameter FCM is effective for analyzing other proliferation-related proteins like c-myc, p53, and Ki-67 in situ.
Impact:
- H-ras p21/DNA dual FCM is a valuable tool for analyzing tumor cell pathophysiology.
- This method can potentially improve prognostic assessment in multiple myeloma.
- The findings contribute to understanding the role of oncogenes in cancer progression.
