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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Visualization of Host-Polerovirus Interaction Topologies Using Protein Interaction Reporter Technology

Stacy L DeBlasio¹, Juan D Chavez², Mariko M Alexander³

¹Boyce Thompson Institute for Plant Research, Ithaca, New York, USA USDA-Agricultural Research Service, Ithaca, New York, USA.

Journal of Virology

|December 15, 2015

Summary

This study introduces protein interaction reporter (PIR) technology to map plant virus-host interactions, revealing complex networks and viral strategies. PIR technology precisely maps these interactions, aiding in understanding plant-pathogen dynamics and developing therapies.

Area of Science:

Virology
Structural Biology
Proteomics

Background:

Demonstrating host-virus protein interactions is crucial but challenging.
Most interactions are complex and difficult to study structurally.
Understanding these interactions is key to virus transmission and host specificity.

Purpose of the Study:

To utilize protein interaction reporter (PIR) technology to map host-pathogen protein interactions.
To elucidate the topological features of these interactions within plant viruses.
To investigate the functional diversity of the Potato leafroll virus (PLRV)-host protein network.

Main Methods:

Infectious Potato leafroll virus (PLRV) preparations were analyzed using PIR technology.
PIR couples a mass spectrometric-cleavable chemical cross-linker with high-resolution mass spectrometry.
Data-derived topological features were identified for cross-linked sites.

Main Results:

PLRV virions exhibit protein interaction 'hot spots' and multifunctional surface topologies.
Modeling, guided by cross-linking data, suggests asymmetric capsid protein packing.
Protein interaction topologies are conserved across viral families (Luteoviridae, Herpesviridae, Adenoviridae).

Conclusions:

PIR technology precisely maps functional host-pathogen protein interaction topologies.
Complex molecular interactions and structural mimicry were identified between PLRV and host proteins.
This work illuminates viral strategies and demonstrates PIR's utility for studying recalcitrant pathogens.

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