In vitro attachment of Salmonella typhimurium to chick ceca exposed to selected carbohydrates

This investigation was designed to study the effect of selected carbohydrates on the in vitro attachment of Salmonella typhimurium to the ceca of chickens 1 and 2 weeks of age. Ceca were surgically removed from chickens immediately after euthanasia, inverted on glass rods, and then rinsed with sterile saline before being exposed to S. typhimurium in a solution of saline containing the carbohydrate to be evaluated. Attachment of S. typhimurium to ceca was reduced in 1-week-old chicks in the presence of N-acetyl-D-galactosamine, L-fucose, D-galactose, L+arabinose, and D+mannose. Minimal or no reduction in attachment of S. typhimurium was noted when ceca from 2-week-old chicks were exposed to the same compounds. Carbohydrates investigated and found to be ineffective for reduction of S. typhimurium attachment to chick ceca were D+fucose and N-acetyl-D-glucosamine.