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Updated: Mar 28, 2026
Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
Published on: January 24, 2025
Molecular characterization of Newcastle disease virus strains isolated from different outbreaks in Northeast India
Barnali Nath1, Nagendra N Barman2, Sachin Kumar1
1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
Abstract:
Newcastle disease virus (NDV) isolates recovered from different outbreaks in chicken flocks in Assam during 2014-15 were genotypically and pathotypically characterized. Nucleotide sequence analysis of fusion (F) and hemagglutinin protein genes showed a close similarity with genotype XIII strains of NDV. Amino acid sequence of F protein showed a virulent cleavage site (112)R-R-Q-K-R-F(117). Furthermore, pathogenicity test in one-day-old chicks and embryonated chicken eggs showed a virulent pathotype of the isolated NDV strains. The study will help us to understand the biology of circulating strains of NDV in Northeastern part of India.
Insights
Newcastle disease virus (NDV) strains from Assam outbreaks were identified as genotype XIII. These virulent NDV strains possess a characteristic fusion protein cleavage site, confirming their pathotype.
Area of Science:
- Veterinary Virology
- Molecular Epidemiology
- Avian Pathology
Background:
- Newcastle disease virus (NDV) poses a significant threat to poultry industries globally.
- Understanding the genetic and pathogenic characteristics of circulating NDV strains is crucial for effective disease control.
Purpose of the Study:
- To genotype and pathotype Newcastle disease virus (NDV) isolates from chicken flocks in Assam, India.
- To characterize the fusion (F) and hemagglutinin (HA) genes of these NDV strains.
- To assess the virulence of NDV strains prevalent in Northeastern India.
Main Methods:
- Nucleotide sequencing of NDV fusion (F) and hemagglutinin (HA) genes.
- Amino acid sequence analysis to identify the F protein cleavage site.
- Pathogenicity testing in one-day-old chicks and embryonated chicken eggs.
Main Results:
- NDV isolates from Assam (2014-15) were genotypically similar to genotype XIII strains.
- Sequence analysis revealed a virulent F protein cleavage site (112)R-R-Q-K-R-F(117).
- Pathogenicity tests confirmed a virulent pathotype for the isolated NDV strains.
Conclusions:
- The circulating NDV strains in Assam belong to genotype XIII and exhibit virulent characteristics.
- This genotypic and pathotypic characterization provides insights into NDV biology in Northeastern India.
- Findings aid in developing targeted strategies for Newcastle disease prevention and control.
