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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
Published on: July 23, 2013
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Application of succulent plant leaves for Agrobacterium infiltration-mediated protein production
1USDA-ARS, Genetic improvement of Fruits and Vegetables Laboratory, 10300 Baltimore Ave, Beltsville, MD 20705, United States.
Journal of Microbiological Methods
|December 15, 2015
Abstract:
When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.

