Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ovarian development is driven by early spatiotemporal priming of the coelomic epithelium.

bioRxiv : the preprint server for biology·2025

Same author

NEDD4 Promotes Sertoli Cell Proliferation and Adult Leydig Cell Differentiation in the Murine Testis.

Endocrinology·2025

Same author

Gonadal sex determination in vertebrates: rethinking established mechanisms.

Development (Cambridge, England)·2025

Same author

Editorial: Proceedings of the 9th international symposium on the biology of vertebrate sex determination 2023.

Frontiers in cell and developmental biology·2024

Same author

Testicular sex cord-stromal tumors in mice with constitutive activation of PI3K and loss of Pten.

Carcinogenesis·2024

Same author

A Human Homozygous <i>HELQ</i> Missense Variant Does Not Cause Premature Ovarian Insufficiency in a Mouse Model.

Genes·2024

Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026

Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026

Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026

Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026

Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026

Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 28, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Non-coding RNAs: An Introduction.

Jennifer X Yang¹, Raphael H Rastetter¹, Dagmar Wilhelm²

¹Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, 3800, Australia.

Advances in Experimental Medicine and Biology

|December 15, 2015

Summary

This summary is machine-generated.

Regulatory RNAs, like microRNAs, have emerged as crucial players beyond messenger roles. This chapter introduces key non-coding RNA classes, their biogenesis, and functions in biological processes.

Keywords:

Endo-siRNAs Gene regulation Long non-coding RNAs MicroRNAs piRNAs snoRNAs

More Related Videos

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Related Experiment Videos

Last Updated: Mar 28, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Traditionally, RNA's primary role was mRNA, tRNA, and rRNA in protein synthesis.
The discovery of microRNAs revolutionized understanding, revealing RNA's regulatory potential beyond coding.
This led to the identification of diverse non-coding RNA classes.

Purpose of the Study:

To provide an overview of current knowledge on major non-coding RNA classes.
To summarize the biogenesis and mechanisms of action for these regulatory RNAs.
To highlight their roles in various biological processes.

Main Methods:

Review of existing literature on non-coding RNAs.
Synthesis of information on expression, biogenesis, and function.
Categorization of different non-coding RNA types.

Main Results:

Identification of multiple non-coding RNA classes, including microRNAs, endo-siRNAs, piRNAs, and lncRNAs.
Significant progress in understanding their diverse biological roles.
Elucidation of their complex biogenesis pathways and functional mechanisms.

Conclusions:

Non-coding RNAs are integral to cellular regulation, impacting numerous biological processes.
Continued research is essential for a comprehensive understanding of their roles.
This chapter serves as an introduction to the dynamic field of regulatory RNAs.