Related Experiment Video
Updated: Mar 28, 2026

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
Published on: March 29, 2019
Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem
Cory J Tobin1, Elliot M Meyerowitz2,3
1Division of Biology and Biological Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA, 91125, USA. ctobin@caltech.edu.
Abstract:
Cells in the Arabidopsis shoot apical meristem are small and divide frequently throughout the life-time of the organism making them good candidates for studying the mechanisms of cell division in plants. But tracking these cell divisions requires multiple images to be taken of the same specimen over time which means the specimen must stay alive throughout the process. This chapter provides details on how to prepare plants for live imaging, keep them alive and growing through multiple time points, and how to process the data to extract cell boundary coordinates from three-dimensional images.
Related Concept Videos
Primary and Secondary Growth in Roots and Shoots
Determining the Plane of Cell Division
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
Determining the Plane of Cell Division
Animal and Plant Cell Structure
Cell Division
Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during...
Morphogenesis

