Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bile acid retention in efferocytic macrophages shapes their inflammatory status during cholangitis.

The Journal of experimental medicine·2026

Same author

Beyond intraocular pressure: introducing a novel glaucoma-specific PROM for evaluating outcomes after IStent inject implantation combined with phacoemulsification cataract surgery.

Scientific reports·2026

Same author

Outcomes of Ab Interno 63 µm vs. 45 µm XEN<sup>®</sup> Gel Stent in Open-Angle Glaucoma: A Five-Year Follow-Up Study.

Journal of clinical medicine·2026

Same author

Assessment of automated loop-mediated isothermal amplification-(LAMP-)based xenomonitoring for Plasmodium spp. in Anopheles mosquitoes.

Malaria journal·2026

Same author

CD160-competent ILC2 are crucial for the ejection of intestinal helminths by the innate immune system.

Mucosal immunology·2026

Same author

Extracellular Vesicle Properties and Functions Are Defined by the Originating Cell's Fitness Status.

Journal of extracellular biology·2026

Same journal

Correction: Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases.

Transgenic research·2026

Same journal

Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective.

Transgenic research·2026

Same journal

Mammalian growth factors enhance regeneration in transgenic tomato lines.

Transgenic research·2026

Same journal

Transgenic expression of human signal regulatory protein alpha (SIRPα) in BALB/c Rag2<sup>null</sup>/Jak3<sup>null</sup> immunodeficient mice enhances human lymphoma xenografts by reducing phagocytosis.

Transgenic research·2026

Same journal

Evaluation of different mutagens in tuberose (Polianthes tuberosa) cut flower.

Transgenic research·2026

Same journal

Suppressor activity against cosuppression conferred by the petunia vein clearing virus genome.

Transgenic research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 28, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

A transgenic, visual screenable marker for soybean seeds.

Lisa Kanizay^1,2, Thomas Jacobs^1,2, C Nathan Hancock³

¹Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.

Transgenic Research

|December 15, 2015

Summary

This summary is machine-generated.

Researchers developed a new method to create dark-colored soybean seeds using a transgenic marker. This technique bypasses natural gene silencing, enabling easier tracking of genetically modified soybeans.

Keywords:

Chalcone synthase RNA silencing Seed coat Soybean Visual screening

More Related Videos

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Related Experiment Videos

Last Updated: Mar 28, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Area of Science:

Plant Biotechnology
Genetics
Molecular Biology

Background:

Soybean seeds are typically buff-colored due to a seed coat-specific small interfering RNA (siRNA) mechanism.
This natural phenomenon limits visual markers in genetically modified soybeans.

Purpose of the Study:

To develop a novel maternal transgenic marker for soybeans.
To overcome the limitations of endogenous gene silencing for visual screening.

Main Methods:

Engineered expression of a rice chalcone synthase transgene.
Designed the transgene with minimal DNA sequence homology to soybean siRNAs to evade silencing.
Observed the resulting seed coat phenotype.

Main Results:

Transgenic soybeans expressing the rice chalcone synthase exhibited dark-colored seed coats.
The dark coloration resulted from anthocyanin pigment production.
The phenotype was confined to the seed coat and did not affect other soybean tissues.

Conclusions:

A novel strategy was developed to produce an easily scorable transgenic marker in soybeans.
This approach facilitates high-throughput screening and analysis of transgenic soybean events.
The method leverages anthocyanin production via a silenced transgene to create a distinct visual marker.