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Substrate structural requirements of Schizosaccharomyces pombe RNase P
D Drainas1, S Zimmerly, I Willis
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
FEBS Letters
|July 17, 1989
Abstract:
RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNA(Ser) genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.