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Updated: Mar 28, 2026

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
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Simultaneous detection of single-nucleotide polymorphisms in a DNA bulge structure using a fluorine-modified
Takashi Sakamoto1, Daisaku Hasegawa1, Kenzo Fujimoto1
1School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan. tsakamo@jaist.ac.jp.
The Analyst
|December 15, 2015
Abstract:
Using 3,5-bis(trifluoromethyl)benzene-modified bisbenzimide H33258, bulged-out nucleobases on a DNA strand can be detected by the chemical shift change in (19)F NMR. Based on this technique, single-nucleotide polymorphisms on a DNA sequence can be analyzed simultaneously.
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