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Related Experiment Video

Updated: Mar 28, 2026

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
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Human bone marrow processing using a new continuous-flow cell separation device.

Martin G Guttridge1, Christopher Bailey1, Christopher Sidders1

  • 1Cellular and Molecular Therapy, NHS Blood and Transplant, Bristol.

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Summary
This summary is machine-generated.

The Spectra Optia effectively reduces bone marrow volume and depletes red blood cells for transplantation. Optimal cell recovery requires minimizing transit time, though refrigeration can extend it up to 24 hours.

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Area of Science:

  • Hematology
  • Transplantation Science
  • Medical Device Technology

Background:

  • Bone marrow processing is crucial for removing incompatible red blood cells (RBCs) or reducing volume before transplantation.
  • The Spectra Optia apheresis system is a potential tool for optimizing bone marrow (BM) preparation.

Purpose of the Study:

  • To evaluate the Spectra Optia apheresis system's efficacy in volume reduction and RBC depletion of human bone marrow (BM) for transplantation.

Main Methods:

  • The Spectra Optia system processed 30 human BM donations (28 allogeneic, 2 autologous) over 12 months.
  • Processing parameters followed manufacturer guidelines, with mean BM collection volume of 1094 mL and RBC volume of 374 mL.

Main Results:

  • Achieved 93.0% volume reduction and 98.8% RBC depletion.
  • Recovered 79.12% mononuclear cells, 88.36% CD34+ cells, and 79.84% CD3+ cells.
  • Transit time and BM age impacted cell recovery; refrigerated transit up to 24 hours was feasible without compromising processing.

Conclusions:

  • The Spectra Optia system effectively performs volume reduction and RBC depletion on human BM for transplantation.
  • Minimizing transit time is recommended for optimal cell recovery, with refrigeration offering a buffer for extended periods.