Related Experiment Videos
Insulin action on different smooth muscle preparations.
V M Altan1, Y Oztürk, N Yildizoğlu-Ari
1Department of Pharmacology, Faculty of Pharmacy, University of Ankara, Turkey.
General Pharmacology
|January 1, 1989
Summary
Insulin inhibits smooth muscle contractions in the gut and vas deferens. Its relaxing effect on the duodenum may involve prostanoid metabolism and calcium pump activation.
Area of Science:
- Pharmacology
- Gastroenterology
- Endocrinology
Background:
- Insulin's role in diabetic gastrointestinal complications is suggested.
- This study investigates insulin's acute effects on non-diabetic animal smooth muscles.
Purpose of the Study:
- To examine the short-term impact of insulin on isolated smooth muscle preparations.
- To elucidate the mechanisms underlying insulin's smooth muscle effects.
Main Methods:
- Isolated guinea-pig ileum, rat stomach fundus, and rat vas deferens contractions were measured.
- Insulin's effects were assessed in the presence and absence of various pharmacological agents.
- Dose-dependent relaxation of rat duodenum by insulin was analyzed.
Main Results:
- Insulin non-competitively inhibited acetylcholine-, histamine-, serotonin-, noradrenaline-, and tyramine-induced contractions.
- Insulin induced a dose-dependent relaxation of the rat duodenum.
- Insulin's relaxing effect was sensitive to sodium orthovanadate, trifluoperazine, verapamil, aspirin, and dexamethasone.
Conclusions:
- Insulin's smooth muscle inhibitory and relaxing effects are linked to prostanoid metabolism.
- Insulin may exert these effects via activation of Ca2+-pump ATPase(s).