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Acute inflammatory effects of a monocyte-derived neutrophil-activating peptide in rabbit skin
S J Foster1, D M Aked, J M Schröder
1ICI Pharmaceuticals, Cheshire U.K.
Abstract:
The inflammatory effects of a monocyte-derived neutrophil-activating peptide (MONAP), purified to homogeneity from lipopolysaccharide-stimulated human peripheral blood monocytes, have been evaluated in rabbit skin. Intradermal injection of MONAP alone caused a mild infiltration of polymorphonuclear leucocytes (PMNL) but did not induce any change in plasma extravasation. When combined with prostaglandin E2(PGE2), MONAP caused a marked and synergistic increase in PMNL infiltration and plasma extravasation into the injected skin sites. The increase in vascular permeability induced by MONAP depended on the presence of circulating PMNL. MONAP is a novel cytokine with pro-inflammatory properties and may have physiological and pathophysiological roles in health and disease.
Insights
Monocyte-derived neutrophil-activating peptide (MONAP) causes inflammation. MONAP combined with prostaglandin E2 (PGE2) synergistically increases inflammatory responses, indicating a role in disease.
Area of Science:
- Immunology
- Inflammation Research
Background:
- Monocyte-derived neutrophil-activating peptide (MONAP) is a cytokine derived from monocytes.
- Its role in inflammatory processes requires further elucidation.
Purpose of the Study:
- To evaluate the inflammatory effects of MONAP in a rabbit skin model.
- To investigate the synergistic effects of MONAP with prostaglandin E2 (PGE2).
Main Methods:
- Purification of MONAP from lipopolysaccharide-stimulated human peripheral blood monocytes.
- Intradermal injection of MONAP and PGE2 in rabbit skin.
- Assessment of polymorphonuclear leucocyte (PMNL) infiltration and plasma extravasation.
Main Results:
- MONAP alone induced mild PMNL infiltration but no plasma extravasation.
- Combined MONAP and PGE2 significantly increased PMNL infiltration and plasma extravasation.
- The vascular permeability increase was dependent on circulating PMNL.
Conclusions:
- MONAP is a novel pro-inflammatory cytokine.
- MONAP, particularly in conjunction with PGE2, plays a significant role in acute inflammation.
- MONAP may have implications in physiological and pathophysiological conditions.