Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microvascular endothelial cell culture.

M A Karasek1

  • 1Department of Dermatology, Stanford University School of Medicine, California 94305.

The Journal of Investigative Dermatology
|August 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progress in our understanding of the biology of psoriasis.

Cutis·1999
Same author

Interleukin-1 induces major phenotypic changes in human skin microvascular endothelial cells.

Journal of cellular physiology·1997
Same author

Persistence of T-cell clones in psoriatic lesions.

Archives of dermatology·1997
Same author

Interleukin-10 induces E-selectin on small and large blood vessel endothelial cells.

The Journal of experimental medicine·1996
Same author

Differential activation of protein kinase C isozymes by phorbol ester and collagen in human skin microvascular endothelial cells.

The Journal of investigative dermatology·1996
Same author

CD8+ T-cells in psoriatic lesions preferentially use T-cell receptors V beta 3 and/or V beta 13.1 genes.

Annals of the New York Academy of Sciences·1995

Researchers developed a new cell culture medium to study microvascular endothelial cells, crucial for inflammation and cancer. This method supports cell growth and function, aiding research into these vital biological processes.

Area of Science:

  • Cell Biology
  • Vascular Biology
  • Biomedical Research

Background:

  • Microvascular endothelial cells are critical for key physiological and pathological processes, including inflammation, tumor metastasis, and wound healing.
  • Studying these cells in vitro is essential for understanding their complex roles.
  • Existing methods for culturing microvascular endothelial cells have limitations.

Purpose of the Study:

  • To review existing methods for studying microvascular endothelial cells in vitro.
  • To introduce a novel modified Iscove's medium for enhanced endothelial cell culture.
  • To demonstrate that cultured microvascular endothelial cells retain important cellular markers and functions.

Main Methods:

  • Review of established in vitro techniques for isolating and culturing endothelial cells from various sources (adult skin, neonatal foreskin, animal models).

Related Experiment Videos

  • Description of a new modified Iscove's medium formulation, including specific supplements like maternal serum, cyclic AMP, and nucleosides.
  • Assessment of endothelial cell viability, growth (up to seven passages), and retention of characteristic markers (Weibel-Palade bodies, Factor VIII-associated antigen) in the modified medium.

    • Main Results:

    • The modified medium successfully supported the growth of both adult and neonatal microvascular endothelial cells through multiple passages.
    • Cultured endothelial cells maintained cytologic markers indicative of their origin and phenotype.
    • Key microvascular functions, such as basement membrane formation, angiogenesis, and response to vasoactive agents, were observed in vitro.

    Conclusions:

    • The described modified medium provides a robust system for culturing microvascular endothelial cells.
    • This in vitro model effectively recapitulates essential endothelial cell functions, facilitating research into vascular biology.
    • The method advances the study of inflammation, metastasis, and wound healing by enabling reliable endothelial cell culture.