Related Experiment Video
Updated: Mar 28, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
[Regulation of rsc1285 gene in type III secretion system in Ralstonia solanacearum]
Objective:
Rsc1285 is one of the putative T3SS-regulated factors in Ralstonia solanacearum, and the regulation of Rsc1285 on T3SS and pathogenicity was characterized.
Methods:
The rsc1285 deletion mutants were constructed by homologous recombination and characterized by complementation.
Results:
The rsc1285 mutant was significantly less virulent than the wild-type strain to infect tomato plants. Rsc1285 controls the expression of hrpB and HrpB-regulating genes, but it is dispensable for the expression of hrpG and prhG.
Conclusion:
R. solanacearum uses Rsc1285 to control the T3SS and pathogenicity via a novel pathway, and this finding provides insights into overall infection mode of R. solanacearum.
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