Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and function of renin.

T Inagami1

  • 1Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HS-142-1, a Novel Non-peptide Antagonist for Atrial Natriuretic Peptide Receptor, Selectively Inhibits Particulate Guanylyl Cyclase and Lowers Cyclic GMP in LLC-PK1 Cells.

Bioscience, biotechnology, and biochemistry·2016
Same author

Increased expression of cyclooxygenase-2 in the renal cortex of human prorenin receptor gene-transgenic rats.

Kidney international·2006
Same author

Beyond the G protein: the saga of the type 2 angiotensin II receptor.

Arteriosclerosis, thrombosis, and vascular biology·2005
Same author

Metalloprotease-dependent ErbB ligand shedding in mediating EGFR transactivation and vascular remodelling.

Biochemical Society transactions·2003
Same author

The renin-angiotensin system in the twenty-first century.

Blood pressure·2002
Same author

Dual effects of angiotensin II type 2 receptor on cardiovascular hypertrophy.

Trends in cardiovascular medicine·2001
Same journal

Effect of melatonin, captopril, spironolactone and simvastatin on blood pressure and left ventricular remodelling in spontaneously hypertensive rats.

Journal of hypertension. Supplement : official journal of the International Society of Hypertension·2009
Same journal

Hypertension and kidney alterations in rat offspring from low protein pregnancies.

Journal of hypertension. Supplement : official journal of the International Society of Hypertension·2009
Same journal

Comparison of the effects of indapamide and captopril on the development of spontaneous hypertension.

Journal of hypertension. Supplement : official journal of the International Society of Hypertension·2009
Same journal

The protective effect of irbesartan in rats fed a high fat diet is associated with modification of leptin-adiponectin imbalance.

Journal of hypertension. Supplement : official journal of the International Society of Hypertension·2009
Same journal

Chronic antioxidant therapy fails to ameliorate hypertension: potential mechanisms behind.

Journal of hypertension. Supplement : official journal of the International Society of Hypertension·2009
Same journal

Endothelial dysfunction in spontaneously hypertensive rats: focus on methodological aspects.

Journal of hypertension. Supplement : official journal of the International Society of Hypertension·2009
See all related articles

Researchers elucidated the primary structure of renin, revealing its active site and homology with aspartyl proteases. Human renin

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • The primary structure of renin is crucial for understanding its function.
  • Renin shares sequence homology with other aspartyl proteases.
  • Characterizing renin's active site is key to its functional understanding.

Purpose of the Study:

  • To determine the primary structure of renin.
  • To identify functional groups within the renin active site.
  • To investigate the unique properties of human renin compared to non-primate renin.

Main Methods:

  • Purification of renin.
  • Determination of primary amino acid sequence.
  • Analysis of active site structure and functional groups.
  • Comparative analysis of human and non-primate renin.

Related Experiment Videos

  • Synthesis of specific inhibitor peptides.

    • Main Results:

    • The primary structure of renin was elucidated.
    • Functional groups in the active site were identified.
    • Sequence homology between renin and aspartyl proteins was confirmed.
    • A long subsite structure in the active site was demonstrated.
    • Human renin exhibited unique characteristics, lacking immuno-crossreactivity and substrate specificity with non-primate renin.
    • A human renin-specific inhibitor peptide was synthesized.

    Conclusions:

    • Elucidation of renin's primary structure is foundational for understanding its complete structure and function.
    • Identification of active site functional groups and sequence homology are key to characterizing renin.
    • The unique active site of human renin was confirmed.
    • Specific inhibitors can be developed for human renin.