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In vitro evaluation of thio-poly acrylic acid for intraoral delivery
Flavia Laffleur1, Nina Leder1, Jan Barthelmes1
1a Department of Pharmaceutical Technology , Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck , Innsbruck , Austria.
Context:
Intraoral drug delivery as mucosal delivery pathway provides a huge platform in the pharmaceutical field.
Objective:
Combining mucoadhesiveness and controlled release of thio-poly acrylic acid as advanced excipient for buccal drug delivery.
Materials And Methods:
Mediated by carbodiimide, cysteine was covalently attached to poly acrylic acid. This thiomer was assessed with regard to cytotoxicity, stability, mucoadhesion, and rheology as well as release behavior of Lidocaine.
Results:
Stability assays of thio-poly acrylic acid were complying with United States Pharmacopeia requirements. Mucoadhesion assay such as tensile (total work of adhesion), bioadhesion, rotating cylinder revealed as this thiomer was superior in comparison to non-thiolated poly acrylic acid with 7.61-fold, 2.8-fold, 5.61-fold improvement, respectively without any toxic effect. The cell viability exhibited over 90% after incubation time of 3 h and 24 h respectively. Lidocaine release showed 1.98-fold more controlled release over 3 h in comparison to unmodified poly acrylic acid.
Conclusion:
Taken the findings in consideration, thio-poly acrylic acid provides excellent stability, controlled release, and superior mucoadhesive features. The prolonged residence time of thio-poly acrylic acid represents a pillar in the buccal drug delivery.
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