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Mushroom tumor: a new disease on Flammulina velutipes caused by Ochrobactrum pseudogrignonense

Zhipeng Wu1, Weihong Peng2, Xiaolan He2

  • 1College of Resources, Sichuan Agricultural University, Chengdu 611130, China Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization, Ministry of Agriculture, Chengdu 610066, China.

FEMS Microbiology Letters
|December 16, 2015
PubMed

Insights

A new bacterial disease, mushroom tumor, caused by Ochrobactrum pseudogrignonense, affects Flammulina velutipes cultivation. This study identifies the pathogen and confirms its role in causing tumors and inhibiting mushroom growth.

Area of Science:

  • Mycology
  • Plant Pathology
  • Bacteriology

Background:

  • Mushroom tumors are a significant disease affecting off-season Flammulina velutipes cultivation.
  • The specific causal organism responsible for this disease has remained unidentified.

Purpose of the Study:

  • To identify the pathogen causing mushroom tumors in F. velutipes.
  • To confirm its pathogenicity using Koch's Postulates.
  • To characterize the pathogen and study its distribution.

Main Methods:

  • Morphological, physiological, biochemical, and molecular characterization of the pathogen.
  • Koch's Postulates were applied to confirm pathogenicity.
  • 16S rRNA gene sequencing for molecular identification.

Main Results:

  • The mushroom tumor disease is caused by a novel bacterial pathogen, Ochrobactrum pseudogrignonense.
  • This bacterium forms tumor-like structures on the substrate surface.
  • It inhibits the formation of primordia and fruiting bodies in F. velutipes.
  • Molecular analysis confirmed the pathogen's close relation to the Ochrobactrum genus.

Conclusions:

  • Ochrobactrum pseudogrignonense is identified as the causal agent of mushroom tumors in F. velutipes.
  • This represents the first documented instance of Ochrobactrum species acting as a mushroom pathogen.
  • The findings provide crucial insights into managing this disease in commercial cultivation.
Keywords:
bacterial pathogenoff-season cultivationsouthwest Chinawinter mushroom

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