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Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Related Experiment Video

Updated: Mar 28, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Quantitative DNA Methylation Profiling in Cancer.

Ole Ammerpohl1, Andrea Haake2, Julia Kolarova2

  • 1Institute of Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 24, Kiel, 24105, Germany. oammerpohl@medgen.uni-kiel.de.

Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2015
PubMed
Summary
This summary is machine-generated.

Epigenetic alterations, like DNA methylation changes, drive cancer development and metastasis. Bisulfite pyrosequencing quantifies these DNA methylation patterns in tumors to identify aberrant epigenetic modifications.

Keywords:
Bisulfite pyrosequencingDNA methylation profilingEpigenetics

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Epigenetic mechanisms, particularly DNA methylation, are crucial for regulating gene expression.
  • Aberrant epigenetic alterations are implicated in the development, evolution, and metastasis of malignant tumors.
  • These changes can lead to phenotypic diversity within cancer cells originating from a single tumor.

Purpose of the Study:

  • To describe bisulfite pyrosequencing as a technology for quantitative DNA methylation analysis.
  • To demonstrate the application of this technology in detecting aberrant DNA methylation in malignant tumors.

Main Methods:

  • Bisulfite pyrosequencing was employed for quantitative DNA methylation analysis.
  • The method allows for precise measurement of methylation levels at specific CpG sites.

Main Results:

  • The study describes the utility of bisulfite pyrosequencing for detecting aberrant DNA methylation.
  • This technique enables the identification of specific methylation patterns associated with malignancy.

Conclusions:

  • Bisulfite pyrosequencing is an effective tool for quantitative DNA methylation analysis in cancer research.
  • Detecting aberrant DNA methylation using this method can aid in understanding tumor development and evolution.