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Updated: Mar 28, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
No evidence for viral sequences in five lepidic adenocarcinomas (former "BAC") by a high-throughput sequencing
Nicolas Berthet1,2,3, Lionel Frangeul4, Ken André Olaussen5,6,7
1Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France. nicolas.berthet@ird.fr.
Background:
The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample.
Results:
None of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses.
Conclusions:
In conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma.
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