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[Pain of high-throughput screening--pan assay interference compounds]
Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|December 17, 2015
Summary
Pan assay interference compounds (PAINS) disrupt drug screening assays. Identifying and removing these problematic molecules is crucial for improving high-throughput screening efficiency and hit rates in drug discovery.
Area of Science:
- Medicinal Chemistry
- Drug Discovery
- Biochemistry
Background:
- High-throughput screening (HTS) is essential for identifying novel drug leads against validated targets.
- HTS campaigns often yield numerous false positives due to problematic chemical entities.
- Pan assay interference compounds (PAINS) are a major class of molecules that interfere with various assay formats.
Purpose of the Study:
- To define and categorize Pan assay interference compounds (PAINS).
- To review the mechanisms by which PAINS interfere with drug screening assays.
- To outline strategies for mitigating the impact of PAINS in HTS campaigns.
Main Methods:
- Literature review of PAINS.
- Analysis of PAINS interference mechanisms.
- Consultation of PAINS classification and countermeasures.
Main Results:
- PAINS exhibit diverse interference modes, including biochemical assay disruption, protein modification, and aggregation.
- Effective identification and removal of PAINS are critical for reliable screening outcomes.
- Strategies exist to manage PAINS, enhancing the accuracy of HTS.
Conclusions:
- Understanding PAINS is vital for optimizing HTS processes.
- Implementing countermeasures against PAINS improves the quality of identified hit compounds.
- This review provides guidance for more effective HTS and drug discovery efforts.
