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Cancer Patient-Derived Circulating Microparticles Enhance Lung Metastasis in a Rat Model: Dual-Source CT, Cellular,
Sheung-Fat Ko1, Hon-Kan Yip2, Yen-Yi Zhen3
1Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung District, Kaohsiung, 833, Taiwan. sfa.ko@msa.hinet.net.
Molecular Imaging and Biology
|December 17, 2015
Summary
Lung cancer patient-derived circulating microparticles (LCC-MPs) were found to accelerate metastatic lung tumor growth and enhance angiogenesis in a rat model. These findings suggest LCC-MPs play a role in promoting cancer progression.
Area of Science:
- Oncology
- Cell Biology
- Medical Imaging
Background:
- Metastatic lung cancer remains a significant clinical challenge.
- Circulating microparticles (MPs) are emerging as key players in cancer progression.
- Lung cancer patient-derived circulating microparticles (LCC-MPs) role in metastasis is not fully understood.
Purpose of the Study:
- To investigate the hypothesis that LCC-MPs enhance metastatic lung tumors.
- To evaluate the effect of LCC-MPs on tumor growth and angiogenesis in a rat model.
Main Methods:
- N1S1-induced pulmonary metastatic hepatocellular carcinoma (HCC) rat model.
- Dual-source CT (DSCT) for tumor monitoring on days 10, 15, and 20.
- Immunohistofluorescence and Western blotting for cellular and molecular analyses.
Main Results:
- LCC-MP-treated rats showed significantly more and larger metastatic tumors compared to controls on DSCT.
- Enhanced angiogenesis was observed in LCC-MP-treated rats, indicated by increased microvessel density (CD34+) and expression of pro-angiogenic factors (VEGF, eNOS, angiopoietin, CD31).
- Increased CXCR4+ and VEGF+ cells were noted in LCC-MP-treated tumors.
Conclusions:
- LCC-MPs promote oncogenic stimulation and accelerate metastatic HCC growth in the rat lung.
- LCC-MPs enhance tumoral angiogenesis, contributing to tumor progression.
