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Quantifying statistical relationships between commonly used in vitro models for estimating lead bioaccessibility.

Kaihong Yan^1,2, Zhaomin Dong^1,2, Yanju Liu^1,2

¹Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE), Mawson Lakes, SA, 5095, Australia.

Environmental Science and Pollution Research International

|December 17, 2015

Summary

Lead (Pb) bioaccessibility in contaminated soils varies significantly across five common in vitro methods. Statistical analysis revealed comparable results between Relative Bioavailability Leaching Procedure (RBALP) and other methods, aiding standardization efforts.

Keywords:

Bioaccessibility Correlations In vitro models Lead

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Area of Science:

Environmental Chemistry
Toxicology
Soil Science

Background:

Estimating lead (Pb) bioaccessibility in contaminated soils is crucial for risk assessment.
Existing in vitro methods for Pb bioaccessibility assessment yield variable results.
Method variability is influenced by extractant composition and soil characteristics.

Purpose of the Study:

To statistically quantify the relationships between five commonly used in vitro methods for Pb bioaccessibility.
To compare Pb bioaccessibility results across different in vitro models using diverse contaminated soils.
To provide a scientific basis for standardizing in vitro bioaccessibility measurements.

Main Methods:

Ten long-term Pb-contaminated soils from Western and South Australia were analyzed.
Five in vitro methods were employed: Relative Bioavailability Leaching Procedure (RBALP), unified BioAccessibility Research Group Europe (BARGE) method (UBM), Solubility Bioaccessibility Research Consortium assay (SBRC), Physiologically Based Extraction Test (PBET), and in vitro Digestion Model (RIVM).
Statistical regression analyses were performed to compare method outputs.

Main Results:

Pb bioaccessibility varied from 1.9–106% (gastric phase) and 0.2–78.6% (intestinal phase) across all methods and soils.
Regression analysis indicated comparable Pb bioaccessibility between RBALP and SBRC, and RBALP and RIVM (0.06g soil).
Slopes between RBALP and UBM (1.21), and RBALP and RIVM (0.6g soil) (1.02) suggest varying degrees of agreement.

Conclusions:

Significant variations in Pb bioaccessibility underscore the importance of method selection.
The chosen in vitro method for bioaccessibility assessment requires validation against in vivo studies for accurate risk prediction.
Findings support the standardization of in vitro bioaccessibility measurements and provide a basis for relating Pb bioavailability to soil properties.