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Updated: Oct 1, 2026

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
Published on: January 17, 2014
A rapid PCR assay to characterize the intact pks15/1 gene, a virulence marker in Mycobacterium tuberculosis
Roberto Zenteno-Cuevas1, Rodrigo Javier Hernandez-Morales1, Lucia Monserrat Pérez-Navarro2
1Instituto de Salud Pública, Universidad Veracruzana, Veracruz, Mexico.
Abstract:
Intact pks15/1 is involved in the biosynthesis of phenolic glycolipids and proposed as a marker for virulence and phylogeny in tuberculosis. Identification of intact condition is achieved mainly by DNA sequencing. For this reason the aim of this study was to develop a reproducible endpoint PCR-assay to characterize it.
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