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Abstract:
Bovine serum albumin was labelled with a terbium complex by means of a reagent prepared from diethylenetriaminepentaacetic acid or diethylenetriaminepentaphosphonic acid and p-aminosalicylic acid. The ternary mixed ligand complexes showed strong fluorescence and were stable in very dilute solution (10(-10)M). The fluorescence of the conjugated products was compared with regard to their application in the fluoroimmunoassay of bovine serum albumin.